Culture medium pH is influenced by basal medium, carbohydrate source, gelling agent, activated charcoal, and medium storage method
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چکیده
منابع مشابه
Adenovirus-mediated gene transfer to adult mouse cardiomyocytes is selectively influenced by culture medium.
BACKGROUND As development of cardiac gene therapies progresses, virally mediated genetic manipulations in cultured cardiomyocytes has become an important experimental approach. While adenovirus (Ad)-mediated gene transfer to neonatal and adult rat cardiomyocytes is well established, viral transduction of cultured adult mouse cardiomyocytes (AMCM) has been more difficult. This study was designed...
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A growth medium with a specific oxidation-reduction potential containing peptone, dextrose, sodium succinate, sodium lactate, gelatin, sodium bicarbonate and blue tetrazolium, an indicator dye, in a tris(hydroxymethyl)aminomethane buffer was used to detect the presence of microorganisms in blood. The procedure involved the introduction of blood (and bacteria) into the growth medium with the dye...
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Neurobasal defined culture medium has been optimized for survival of rat embryonic hippocampal neurons and is now widely used for many types of primary neuronal cell culture. Therefore, we were surprised that routine medium exchange with serum- and supplement-free Neurobasal killed as many as 50% of postnatal hippocampal neurons after a 4 h exposure at day in vitro 12-15. Minimal Essential Medi...
متن کاملThe Role of Activated Carbon in Tissue Culture Medium
Tissue culture is an in-vitro technique for replicating a plant without introducing the degree of genetic variability inherent in sexual reproduction. Tissue culture has proven commercially important for the replication of ornamental varieties of house plants and is used extensively in research for applications in forestry. Activated carbon (AC) was initially added to tissue culture media in an...
متن کاملComparison of Mycobacterium tuberculosis culture using liquid culture medium and Lowenstein Jensen medium in abdominal tuberculosis.
BACKGROUND Traditionally, the Lowenstein Jensen (LJ) medium has been used for culturing Mycobacterium tuberculosis. In abdominal tuberculosis (TB), the reported yield from tissue culture is between 20% and 60%. Liquid cultures are reported to give a higher yield but there is little data available in abdominal TB. AIM To compare the yield of TB culture with BACTEC 460TB liquid medium and LJ me...
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ژورنال
عنوان ژورنال: Plant Cell Reports
سال: 1991
ISSN: 0721-7714,1432-203X
DOI: 10.1007/bf00232516